. 1. Select up to 3 products. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. The top- and bottom-reading Gemini EM Microplate. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 314 low lamp level warning. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). 2. Part of a plate can be selected so that only a selected area of the plate. Molecular Devices (UK) Ltd. Get Quote . Site. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Reconstitution of. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. Range of 96, 384-well SBS microplates . 057, depending on lot number. 0% ± 0. It is designed to work seamlessly with a variety of 96 well microplate readers, including popular brands such as Agilent™, Berthold™, Biotek™, BMG LABTECH™, Molecular Devices™, Perkin Elmer™, Promega™, Tecan™, and. Bioz Stars score: 86/100, based on 40 PubMed citations. Ensure that cells reach around 80–90% of confluence on the day of the. Lucid Scientific. Features. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. Capabilities. 384-WELL SBS STERILE MICROPLATE. Thermomax model provides thermal control and vertical shake, perfect for. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 90349). Category 2 and 3 microplate readers use gradient heating as well. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13The microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. Instrument settings can beCorning 96-well black microplates with clear bottom (Corning cat. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. 9000-0756. In the latter case, 8 wells in each column are perfused in series and the 12 columns are. Microplate washers are designed for effortless use with 96- or 384-well plates in routine ELISA applications. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. 0A-Sunnyvale, CA-Made in USAWe offer a range of multiwell plates including 384-well, 96-well and 24-well plates with a selection of membrane types:. Description. Click Add to create the plate. Cross-talk <0. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. The IC50s by cell rounding and LysoTracker. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. 5°C at 37°C. x. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. 00. Mix samples in microplate wells by user-programmable Automix feature. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. The system features 96, 384,. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Wells of 96-well plates (NEST) were pre-coated with 100 μg/ml of collagen solution for 1 hour. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro Software. Optimized for fluorescence microplate readers. to each well. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. Or scan up to 384 wells at any time to determine the best assay conditions. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. More Product Information. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. 5% at 1. 96 wells 384 wells 1 pM 1 pM Optimized bottom sensitivity (fluorescein) 96 wells 384 wells 2 pM 2. Linear, Orbital Slow, and Orbital Fast. Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. 306 plate air cal. This reader can also be used as a standard. Place the microplate in the drawer. Contact our support team with your questions regarding Molecular Devices products and applications. Source plate capacity; 1 x plates. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. The. Some will only support one well density (e. Products : Ionworks HT, Ionworks Quattro. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Description: Enter plate name (optional)(2) Wild‑type KRAS inhibits the migration and invasion of pancreatic cancer through the Wnt/β‑catenin pathway Molecular medicine reports January 1, 2023 Hu X, Zhang R, Yao J, Mu B, Zhao C. 0025 OD from 0. Plates were read in the SpectraMax M5 microplate reader at 485ex/515em with bottom read. Select models. 001 ABS (displayed), 0. No. Sort By: Show:. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty EarlyTox Live/Dead Assay Kit (Molecular Devices cat. Spectramax ® Plus microtiter plate reader (Molecular Devices, Sunnyvale, CA) or similar UV-Vis plate reader; SoftMax ® Pro software (Molecular Devices) (optional) UV compatible quartz plate (for example: # R8024; Molecular Devices) or 96-well Costar ® with UV transparent bottom (for example: #3635 ; Corning Inc. 00. No tools required to assemble. Contents of wells were pipetted up and down several times to mix. Plate formats 6, 12, 24, 48, 96, 384 wells Light source Xenon flash lamp (1 joule/flash) Detector Photomultiplier (R-3896). The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. It features fully-automated kinetic reads of the 96-well plate via the WinKQCL TM Software. #CA961) Methods. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. Gemini EM Reader scans. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . 500 OD ± 3. These microplates have a corner notch for use in standard plate readers. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices, through the purchase of FLIPR Assay Kits. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. This is equivalent to having 511 built-in filters,The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. 0 . x. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. Controlled by the computer. Top reading gives greater sensitivity for 96 well plates, unless you have cells adhering to the bottom of the well. 5A spare fuses, 2 cleaning needles, tubing sets for all bottles, cleaning tool for needles. Published Dec 16, 2020 | Updated Dec 16, 2020. They can be read by most microplate readers. Can read 96 well plates (optional 384 well). * Note: Programming the washer with a final aspirate height of 4. plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well microplates. SpectraMax i3x Multi-Mode Microplate Reader. FlexIII. Click Create. The luminescence was measured in a SpectraMax. A = 2 - log 10 (%T). It features a modular design that allows users to add or change detection cartridges according to their needs. Selects either 96-well or 384-well plate for a stand-alone read. 96-well) while others have flexibility to. It has a 340-750nm Spectral Range. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. 100 fmol 1536-well (8 µL) 0. Materials. Luminescence applications and assays. For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro densitometry of TLC plates and electrophoresis gels. 96-well-plate-scan-time-2-colors-1-site-well. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. Plate chamber design of the SpectraMax category 1 microplate readers. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Features of Streptavidin Coated Plates include: • Approximate binding capacity is ∼5 pmol biotin/well. x. Reader Documentation Version No. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. com. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Unparalleled performance on a personalized platform. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). When the user applies a set temperature of 37°C, the top is heated to 37. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Through absorbance readings from both the ultraviolet and visible spectrum range, it can determine the concentration of samples, conduct positive or negative detection of antibodies and. Quartz 96 Well Plates | 96 Well Plate Flat Bottom | 96 Well Plate Reader | 96 Well Glass. 000 ABS • Resolution: 0. 24-, 96-, 384-well plates and microwell strips; Manifold Channels: 96-well washing: 8-well (1x8) manifold, 2x8-well manifold, 12-well (1x12) manifold, 8-well short tube (1x8) manifold. Related Products: 96 Well Plate Reader. Incubation. 5 µL of each sample was pipetted into a 96-well plate for PCR. 2 pM 384–well: 3 pM 96–well: 2 pM 384–well: 4 pM 1 0200-7012POS Glow for 96-, 384-, and 1536-well plates Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. $3,450. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into cardiomyocytes and neurons, up to 100 times per. 2 OD at 200 nm; this decreased to 0. Please click on the links below to view the Microplate Reader Comparison Tables. HeLa. Bowman Dispensers. Read up to 2 wavelengths in endpoint absorbance for 96-well plates. and glow luminescence modes in 96- to 384-well plates. VMax . NanoQuant plate. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. Molecular Devices: FlexStation 3: Multimodal: Limited - monochronometer based: N/A:the iD5 reader’s filter system. . explore more. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. Enter the name of the plate type i. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution forThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. The StakMax stacker also allows users to feed plates into the loading area manually. 1. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). An example of dual-luciferase assay results. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Figure 2-23: Bottle holder To install the bottle holder: 1. 315 can't find zero order. #E010) Pyroplate 96-well microplate (ACC cat. We would like to show you a description here but the site won’t allow us. Microplate Reader. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. Two days before the experiments, cells are seeded into the clear-bottom 96-well plates (approximately 10,000 cells per well for cancer cell lines and 30,000–40,000 cells per well for fibroblasts and NPC; see Note 5) and cultured in the media suited for users’ cell lines. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. Opaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. 005 OD Normal and Rapid modes, 96- and 384-well plates Fluorescence Read Timing Excitation Filter: 485/20 nm Emission Filter: 528/20 nm 96-well read: 89 sec. B. Populate the following info under Description. 2b,c. Compare specifications and request a quote from leading suppliers. Shaking: Linear, orbital, and double orbital. measurements in compatible Molecular Devices® microplate readers. QuickSync. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). 5 ng per well. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Answer. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. We would like to show you a description here but the site won’t allow us. Determine concentration using the Beer-Lambert LawPage 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. UV absorbance detection. SoftMax Pro GxP Software version 5. UV absorbance detection. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. Published Dec 16, 2020 | Updated Nov 07, 2022. Contents of wells were pipetted up and down several times to mix. 9000-0756. 190. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. We would like to show you a description here but the site won’t allow us. It features a modular design that allows users to add or change detection cartridges according to their needs. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. Single or dual wavelength read, kinetic readings, automix, endpoint, and blank pattern, curve fits included with PC Software. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. 100 μL of working reagent was added to each well after the 10-minute preincubation step. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. Plate Shaking. 2% in. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 6. 003 fmol 0. Browse for different fluorescent readers below and compare across different instrument suppliers. Our microplate. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. 17,000 cells. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm: Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra: No. flat-bottom: 50-60 µl. 00 . 2. • Gently wash cells once with 200 μl (for 96 well plate) or 400 μl (for a 24 well plate) warm (37°C) 1X DPBS (containing Mg and CaCl 2). White light and fluorescence images of the 6-well plates were captured with the ClonePix 2 System. 313 reference gain check fail. BioTek Instruments Synergy Neo2 Multi-Mode Reader Fluorescence Measuring Equipment. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. Plate types Flat and round 96-well plates Data output Export to PC Detection system Single channel silicon photodiode Reading speed 25 seconds Software SoftMax Pro Software Shaking None Temperature control None Linearity 0. x. Software Validation. 3% in white 96- and 384. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. A. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. maximum-illumination-channels. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Next-generation luminescence plate reader. This offers the potential for fast and convenient measurements of FAAH activity using a 96-well plate spectrophotometer. Compact designs make these instruments. 00. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. In addition to increased throughput, microplate formats enable researchers to miniaturize their analysis (using less sample and reagent) to study regulatory events and. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. #3603) SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. An industry-renowned instrument for monitoring GPCRs and ion channels. 0 . 3% in white 96- and 384-well microplates < 3 x 10-5 (white plates) < 3 x. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. Molecular Devices' microplate readers. Step 2: Launch SoftMax Pro Software. Spectrofluorometer from Molecular Devices® provides a flexible environment. 20 µL (96-well plates) or 10 µL (384. 4 (Molecular Devices cat. (one set per assay) to clear 96-well microplates. Materials. . Never order or replace filters to optimize an assay again!6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. 96 well plate injection time with SpectraMax® L plate reader. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. 1 - 32 of 54 Sort View Show > Well Plate Reader Accessory PerkinElmer For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro. EMax. Now select the area of the plate to read. 0. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). A Costar 24-well tissue culture plate with col1 = blanks and doubling cell a list of plates specifically supported shows, 96, 384, 24, 12, 6 well types. Multimode microplate reader equipped with latest detections like fluorescence,. * Provides sufficient reagent for 10 plates (96-, 384-well) R8128 FLIPR Membrane Potential Assay Kit Blue (Explorer) (10) vials Component A (blue)* (1) buffer bottle. Multimodal plate reader: Yes: 25 mm: Yes—Max. Selects either 96-well or 384-well plate for a stand-alone read. On the day of the assay, the plate was equilibrated. It offers extremely low absorbance background, excellent. Fluorescence, Absorbance Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec Temperature Control: Ambient +5C up to 42C Shaking: Linear and Orbital. The SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . The same volume of genotyping mixture (2x KASP Master mix plus KASP Assay mix) was added to the each well. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. Absorbance was then determined at 450 nm by SpectraMax Paradigm microplate reader (Molecular. Molecular Devices, LLC Plate Formats: 96 or 384 Well Plates; Manifold Channels: Inquire; Dispensing Liquids: Inquire; Citations: Get Quote. When a. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. White 96-well platePlate formats. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. These lab workhorses are used in drug discovery. (Molecular Devices cat. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMolecular Probes® 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and autofluorescence and so are optimized for fluorescence-based applications. x. (96- and 384-well with pipetting) 6-384 (96- and 384-well). 46Click the Read button in the SoftMax Pro3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact. Exposure of ApNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 382 nm). ZERO BIAS - scores, article reviews, protocol conditions and moreThe VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. x. U-bottom: 30-40 µl. Therefore you need to ask a physicist. Published Dec 16, 2020 | Updated Dec 16,. Cayman Chemical. 000 to 2. Molecular Devices LLC Applications: Inquire; Plate Formats: 96-, 384- well. Published Dec 16, 2020 | Updated Dec 06, 2021. D. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. An entire 96-well plate can be processed in less than six seconds. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. SpectraTest FL1 Fluorescence Validation Plate User Guide Molecular DevicesData are shown as the mean ± s. Supplier: Molecular Devices. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. x. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume differences that may.